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Image Search Results
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Lysosomal trafficking regulator restricts intracellular growth of Coxiella burnetii by inhibiting the expansion of Coxiella -containing vacuole and upregulating nos2 expression
doi: 10.3389/fcimb.2023.1336600
Figure Lengend Snippet: LYST inhibits the intracellular growth of C . burnetii by reducing CCVs expansion. (A) HeLa cells were transfected by different siRNAs, and CCVs (Green) were detected at 5 days post infection; (B) HeLa cells were transfected by different siRNAs, and CCV diameters were compared at 5 days post infection. Data are representative of two independent experiments and bars represent the mean ± SD. *, p<0.05, ****, p<0.0001.C. HeLa cells were transfected with siRNAs for 2 days and then pretreated with E-64d, following by infected with C . burnetii , CCVs (green) in HeLa cells were stained at 5 days post infection; (D) HeLa cells were transfected with siRNAs for 2 days and then pretreated with E-64d, CCV diameters were measured. Data are representative of two independent experiments and bars represent the mean ± SD. ****, p<0.0001; (E) HeLa cells were transfected with siRNAs for 2 days and then pretreated with E-64d, intracellular growth curves of C . burnetii were analyzed. Data are representative of three independent experiments and bars represent the mean ± SD. *, p<0.05, ***, p<0.001 in comparison with control group at the day 7.
Article Snippet:
Techniques: Transfection, Infection, Staining, Comparison, Control